Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. Monday to Friday 8am - 5pm GMT. SpectraMax i3x 多功能微孔板读板机 SpectraMax Paradigm 多功能微孔板读板机 SpectraMax M5e 多功能微孔板读板机 扫一扫关注我 们的官方微信 Molecular Devices 大中华区 全国咨询服务热线: 400-820-3586 Email: info. The calculated LOQ values were 0. 0´ 104 1. A 1:2 dilution series of BSA was prepared in PBS, starting at 100 mg/mL, in LoBind microcentrifuge tubes. Both readers demonstrated their ability to detect the HTRF signal from the terbium donor and red acceptor combination, and they could be used for saturation studies using the Tag-lite technology. (3) Molecular Devices, SpectraMax i3x, Microplate Readers (2) 2022 (1) 2020 Invetech Korus KRS-100 Elutriation & Washing Systems (3) 2021 ThermoFisher Scientific, invitrogen, CytKick Max AutoSampler w/ Attune NxT: Acea NovoCyte Flow Cytometer: Appliedbiosystems, QuantStudio 7 Flex, Real-Time PCR SystemThe SpectraMax i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. . The M5 can read volumes down to 2uL with the uMax Low. Find regional support. 0001 0. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Microplate optimization should be performed the first time the assay is run, and subsequently for . Table 3 Optimal instrument settings for Valita Titer Plus assay Fluorescence Polarization measurement on Molecular Devices microplate readers. . Antigen Quantitation Protocol. ZERO BIAS - scores, article reviews, protocol conditions and moreThe SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. Seller Details. Reaction catalyzed by the firefly luciferase. The plate was covered and incubated for one hour at room temperature. Subsequently, 100 μL of the substrate solution (2 mM of p-nitrophenyl butyrate in dimethylformamide) was added into the mixture, and incubated at 37°C for 15 min. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. The e ect of ASC-exosomes on the melanin levels inThe 70% methanolic extract of steamed root (100 µL) was added to 1 mL of diethylene glycol and 10 µL of sodium hydroxide (NaOH). • SpectraMax i3x Injector Cartridge with SmartInject™ Technology • Expand your research capabilities to include flash-based . Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. 1. Description 1. SpectraMax® i3 Multi-ModeDetectionPlatform withtheSpectraMax® MiniMax™ImagingCytometer UserGuide 5025027A April2013. 0. . . Contact Local Team. Data analysis. 2/5 apart, not needed o Otherwise, ensure cutoff is 10nm less than desired emission wavelength. Microplate reader assays requiring infrared (IR) or red-shifted. Ease of use Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. SpectraMaxi3Multi. RIDASCREEN Gliadin competitive ELISA. 1. The chamber. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. Controls containing all reaction. Description. The heating element design of the category 1 microplate readers, such as the SpectraMax i3x Multi-Mode Microplate Reader, applies a dual area sensor for temperature control. Database contains 1 Molecular Devices SpectraMax i3x Manuals (available for free online viewing or downloading in PDF): Installation manual . Molecular Devices社の製品: 吸収度マルチモードマイクロプレートリーダー SpectraMax® i3x に関するすべての情報をご覧ください。. ，高内涵成像分析技巧——3D功能展示1-3D预览、共定位、测距，SpectraMax Mini 多功能微孔板读板机带你自信地迈出第一步！ ，多功能酶标仪 SpectraMax i3x 之动力学检测法的设置，iD5注射器卡盒操作视频 - 20201228_8，SpectraMax iD 系列触控屏 overview，【网络讲堂】高内涵. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. . 因为是导师第一任学生，没有师兄师姐，加上本人悟性也差，小小pcr花了半年时间才终于有点样子。因为经历过，所以懂得，故而分享之，希望能给到屏幕前的你一点点帮助! 如有失误，欢迎指正~最后，不要忘了一键三联哟，谢谢大家！, 视频播放量 6293、弹幕量 2、点赞数 122、投硬币枚数 55、收藏. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. I am a person who can handle a range of laboratory and data management tasks with precision and accuracy. Molecular Devices’ ScanLater Western Blot Detection Cartridge uses TRF detection to turn its SpectraMax i3X and Paradigm plate readers into Western blot readers. china@moldev. SoftMax Pro. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. HTRF IP1 standard curve measured on the SpectraMax i3x (green circles) and SpectraMax iD5 (red circles). Capture Flash Assays with Ease. SpectraMax i3x Register or Sign In. Faster data acquisition and analysis with preconfigured SoftMax Pro protocols. Bioz Stars score: 98/100, based on 1 PubMed citations. Binding and Inhibition. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. The SpectraMax i3x is a multi-mode microplate reader that measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western blot, cell imaging, and fast kinetics. QuickSync. It has a cooled PMT, a no-stain cell analysis algorithm, and a field-upgradeable imaging cytometer. SpectraMax iD3 and iD5 Register or Sign In. SpectraMax i3x Multi-Mode Detection Platform. User-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. Explore PDF for more details. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors; It allows you to explore cellular pathways and protein activation and expression in one system;In this application note, we describe how the SpectraMax ® i3x Multi-Mode Microplate Reader with SpectraMax Injector Module is used to perform calcium assays on two different cell lines. Easily upgrade or switch between detection modes with user-exchangeable modules – just open the drawer and insert the module you want. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. 99 to $1999. It allows you to explore cellular . CHO M1WT3 cells are Chinese hamster ovary cells stably transfected with the M1 muscarinic G q -coupled receptor. . Example: [Drawer]. Multimode Reader Comparison Chart. Overview of Western Blot Cartridge. SeeThe SpectraMax i3x measures monochromator-based absorbance, fluorescence and luminescence with a future-ready platform that can evolve with the researcher’s needs. Biochrom New EX Read 400 Microplate Reader. 3) At the bottom of the strip, click on "Show. 98 / 100 stars. If no sound is heard during initialization, contact technical support. User-installable and removable detection cartridges allow the detector to be configured for specific applications and easily. Filterless readers provide consistent results with steady temperature regulation. 1 software Download page. . Description 1. Find regional support. Settings not required by a reader are indicated by ‘---’. View the Molecular Devices website. SPECTRAMAX i3X, INJECTOR MODULE CARTRIDGE model number 0200-7029. One sensor measures the temperature of the top chamber heating foil, and another is used to measure the bottom chamber heating foil. A: percent GFP-positive cells calculated using the classification feature in the software. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. The SpectraMax i3x reader allows one to unravel the mysteries of science by exploring cellular pathways and protein. 5 µL anti-cAMP-Cryptate. Actual shake speed is based on the microplate format. SpectraMax M5. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. SpectraMaxParadigmMulti-ModeDetectionPlatformUserGuide 4 5014038F Chapter5:ReadModesandReadTypes 71 SupportedReadTypes 71 AbsorbanceReadMode 74 FluorescenceIntensityReadMode 79the SpectraMax Paradigm Multi-Mode Detection Platform User Guide. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellEvery SpectraMax ® Paradigm Detection Cartridge starts with an application optimized excitation energy source either in the form of a long life, high powered LED, xenon flash lamp, or high powered laser. The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate. . Automated for ease of use. As per Volza’s India Import data, Spectramax import shipments in India stood at 365, imported by 145 India Importers from 28 Suppliers. Supported Windows Operating System: See release notes. . 4. Figure 1. 7. Download PDF. SpectraMax iD3 reader with touchscreen and inserted USB flash drive. Both Microplate Optimization and Read Height Adjustment were. Here, we demonstrate the benefits of using the SpectraMax ® i3x Multi-Mode Microplate Reader with the SpectraMax ® MiniMax™ 300 Imaging Cytometer in combination with MetaMorph ® Microscopy Automation and Image Analysis Software for performing automated neurite outgrowth imaging and analysis. 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideDiscover how the automation-friendly SpectraMax® i3x Multi-Mode Microplate Reader, designed to expand research capabilities through its versatile and user-upgradeable application modules, can. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel detection capabilities without the need for service. Spectranet customer portalSpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. The SpectraMax i3x reader offers a modular design allowing the addition of FP detection to the instrument in just minutes. Unlimited breadth of. 312 gain calibration failed. SeeSpectraMax MiniMaxImagingCytometeronpage85. All the readers were operated using SoftMax® Pro Software, which also has built-in data analysis and curve-fitting for automatic calculation of EC 50. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The assay can accurately test the toxicology of compounds as shown in Figure 1. 303 unable to cal dark current. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Spectranet customer portalThe SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. Read modes. 2. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. 6% for the on-board optics (monochromators). The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. SpectraMax M3 Plate Read, Plate Map, Data Analysis and Export Setup pg. Using the SoftMax Pro Workflow Editor, a kinetic cycle containing an absorbance read (PlateOD600) and a fluorescence read (PlateGFPBottom) with a 5-second linear plate shake between reads was created (Figure 1). 012 fmol 0. 315 can't find zero order. *On the SpectraMax i3x reader only. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploringfluorescence polarization, trf, fret, igg quantitation, elisa, western blot, spectramax i3x, spectramax id5 multi-mode microplate reader, spectramax id5, spectramax paradigm, spectramax m5, spectramax m5 multi-mode microplate reader, high-throughput igg quantitation, mab igg titer, softmax pro, valita titer, valitatiterA microplate reader that can detect two or more applications is considered a multi-mode plate reader. Read height should be optimized for . Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. to View Figures. Keywords: htrf spectramax detection cartridge, spectramax i3, spectramax paradigm system, htrf reagents, spectramax i3 and paradigm readers, HTRF assay, htrf. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. . SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. techsupport. The information is below the horizontal line. 304 signal level saturation. MC38 in vivo tumor model in hPD-1. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3, i3x and Paradigm Multi-Mode Readers. Settings not required by a reader are indicated by ‘---’. R8362. Danaher Inc spectramax i3x. Find our technical support information along with contact details. Open the catalog to page 1. BRET2canbeusedtostudyvariouskindsofprotein-proteininteractions. Spectranet customer portal. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Align the two slots on the bottle holder with the two pegs on the rail. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3, i3x and Paradigm Multi-Mode Readers. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. The chamber. 003 fmol 0. SpectraMax® L reader support; ü. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. Supported Windows Operating System: See release notes. Reporter Assay. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). +44-118-944-8000. However, no piece of equipment is immune to breaking down. Cell viability는 세포막 Integrity 또는. SpectraMax ® iD3 and iD5 Multi-Mode . SpectraMax i3x plate reader (Molecular Device, contact supplier directly) Vacuum pump system (e. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. The SpectraMax i3x reader features a cooled photomultiplier tube (PMT) for low background noise in luminescence. In brief, a pre-titrated dose of virus was incubated with 8 serial 5-fold dilutions of serum samples in duplicate in. ISM Equipment Classification (Group 1, Class A) This equipment is. My proficiency in techniques such as •PBMC isolation, counting and. . 1. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. The SpectraMax i3x’s user-upgradeable application modules will future-proof your research to include most assay capabilities of the SpectraMax iD3 and iD5 readers, as well as additional capabilities such as imaging. com. It allows you to explore cellular pathways and protein activation and expression in one system. Molecular Devices Software. Promega. ATP LLD (pM) ATP LLD (moles) SpectraMax i3x, luminescence . The SpectraMax i3x Multi-Mode Detection Platform User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax i3x, a versatile and customizable microplate reader that can perform various assays and imaging tasks. Figure 4. The SpectraMax i3x reader provides theSoftMaxProSoftwareFormulaReferenceGuide 8 5049093A SummaryFormulas Summariesconsistoffourparts: ASummaryname(whichisusedtorefertotheSummaryinotherformulas). The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast. Not compatible with Home Edition. Figure 1. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. . Transcreener® HTS is a universal, high throughput biochemical assay platform based on the detection of nucleotides, which are formed by thousands of cellular enzymes—many of which catalyze the covalent regulatory. SpectraMax i3x Multi-Mode Detection Platform User Guide. 더 많은. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Introduction. Note: Additional readers with similar performance are listed. Molecular Devices SpectraMax i3x Multi-Mode Microplate Platform. 306 plate air cal fail,low light. Shop For Laboratory Equipment featuring a wide variety of new, used and refurbished laboratory equipment. Monday to Friday 7am - 5pm Pacific Time. Additionally, user-installable options allow the SpectraMax i3 System to. HeLa cells were plated at 15,000 cells per well in 100 µL. Results for SpectraMax i3x reader with NanoBRET Detection Module (blue) and on-board luminescence detection mode (orange). ATP (“flash” luminescence using . to View Figures. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 0. Downloads. ValitaTiter (i3x i3) ValitaTiter (iD5) ValitaTiter (M5 M5e Flex3) ValitaTiter (Paradigm) ValitaTiter (FilterMax F5) Assay-Specific. This eBook shows how to see more and do more with an imaging cytometer that enables rapid imaging and cell analysis. Fluorescence measurements of the soil solutions and enzyme activities were made using a microplate reader (SpectraMax i3x, Molecular Devices, USA). The SpectraMax i3x Multi-Mode Microplate Reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologyMeasure oxidative metabolism and glycolytic activity on the SpectraMax i3x reader. ZERO BIAS - scores, article reviews, protocol conditions and more. Expand your lab’s capabilities to include flash applications such as dual luciferase and ATP assays with the SpectraMax® i3x injector module with SmartInject® Technology ensuring equal mixing across the plate for high-precision experiments. Molecular Devices SpectraMax i3x Multi-Mode Detection Platform. STAKMAX. . Tip: MCF-7 cells have a polygonal morphology with irregular dimensions, and they have a habit of clumping into large aggregates. ClonePix 2 Mammalian Colony Picker; QPix Microbial Colony Pickers; CloneSelect Imager; DispenCell Single-cell Dispenser; 3D. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence,. The SoftMax® Touch Software is installed in. Itemploysa luciferaselabel(where“label”generallymeansfusedbytheuseofexpressionvectors)forthe oneproteinandaGreenFluorescentProtein(GFP)variantlabelfortheother. . 312 gain calibration failed. Find regional support. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. SpectraMax® Microplate Readers and SoftMax® Pro Software. Data Analysis. The plate was then read on the SpectraMax iD5, SpectraMax i3x, and SpectraMax M5e readers using the settings in Table 1. TaqMan SNP. Manufacturers try to design their instruments so they can be. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Detection cartridge modularity allows for easy addition of new read modes within minutes without. Eight different automated tests are provided: • Absorbance accuracy (linearity) • Precision (reproducibility) • Stray light • Wavelength accuracy • Wavelength repeatability SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. 99. Unparalleled performance on a personalized platform. The Relative Luminescence Units (RLU) were measured using the SpectraMax i3x Luminescence Glow cartridge (Lum 384) (0200-7015POS, Molecular Devices) and normalized to the cell count or area, which were determined using the Mini MaxTM 300 Imaging Cytometer (5024062, Molecular Devices). . . Spectramax I3x, supplied by Danaher Inc, used in various techniques. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. Prepare your i3/i3x instrument for shipment. spectramax i3x - by Bioz Stars , 2023-11. SpectraMax i3 Install Guide; SpectraMax i3; SpectraMax i3x; SpectraMax iD3; SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. Figure 3. The 4x washing steps were also performed on deck using the Span-8 aspirate and dispense for the first three washes. Get a front row view of your cells. SpectraMax i3xは、吸光・蛍光・発光スペクトル測定のためのマルチモードマイクロプレートリーダーです。. ImageXpress Confocal HT. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. Wait 20 seconds. It allows you to explore cellular pathways and protein activation and expression in one system. Designed specifically for the SpectraMax® i3 Multi-Mode Detection Platform, the field-upgradeable SpectraMax® MiniMax™ 300 Imaging Cytometer enables quick inspection of cells and provides insight into phenotypic changes that are important for understanding cytotoxicity, cell proliferation, and protein expression. The SpectraMax® i3 Multi-Mode Microplate Reader Detection Platform evolves with your future research needs and offers a breadth of application possibilities. SpectraMaxMiniMaxImagingCytometerInstallationGuide 10 5024111D AttachingtheCytometertotheMicroplateReader Prepareaworkareathatisapproximately130 cm(51. 9 x 10-15. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Current methods available to rapidly detect these can be resource intensive and thus sub-optimal. Introduction. 2. The. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening SoftMax® Pro 7. Learn how you can perform flash-based applications, such as dual luciferase and ATP assays, with high precision and accuracy using this cartridge. SpectraMax i3/i3x readers must be equipped with a suitableThe SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. The SpectraMax® i3x offers a wealth of applications; it can measure fluorescence, absorbance and luminescence, and its modular upgrades enable western blotting, imaging cytometry and injectors. 5 decades, with sensitive detection of cells. NORTH AMERICA. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. The SpectraMax i3x Multi-Mode Detection Platform User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax i3x, a versatile and customizable microplate reader that can perform various assays and imaging tasks. Core features include biological and fume hoods, a cold room and multiple freezers, and access to institutional space for radiolabeling and radiation monitoring. ü; ü. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular. Materials and methodsSpectraMax i3x Multi-Mode Microplate Reader. . One sensor measures the temperature of the top chamber heating foil, and another is used to measure the bottom chamber heating foil. Ribonuclease (RNase) R digestion assay For identifying the stabilization of circVRK1, 2 μg isolated RNA from HBMECs was digested by RNase R (3 U/μg; RNR07250, Geneseed, Guangzhou,. Spectramax I3x, supplied by Danaher Inc, used in various techniques. Qualify the absorbance performance of SpectraMax® iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Rutin was used to generate standard curves for quantification. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. With optimized reagents, option to operate the. Table 1. Cell counters for accurate and. each new assay. 1. 9000-0756. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. Supplier: Molecular Devices. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Concentrating the excitation light on the sample through label specific optics, high levels of sensitivity and performance are reached. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. 020 fmol 0. The SpectraMax i3x reader is engineered to perform with Spectral Fusion Illumination for increased sensitivity across the entire wavelength range, and a cooled photomultiplier tube (PMT) for improved detection in extremely. Spectramax I3x, supplied by Danaher Inc, used in various techniques. 660-665 Eskdale Road. Using the SoftMax Pro Workflow Editor, a kinetic cycle containing an absorbance read (PlateOD600) and a fluorescence read (PlateGFPBottom) with a 5-second linear plate shake between reads was created (Figure 1). The corrected absorbance is within 5% of the. . SpectraMax i3x Multi-Mode Detection Platform. The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. SpectraMax i3x and Paradigm readers require the FP-FLUO. Keywords: filtermax multi mode microplate readers, spectramax paradigm multimode detection platform, spectramax paradigm system Created Date: 11/16/2010 12:21:51 PMThe SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. Molecular Devices ScanLater Western Blot Detection Platform. The treatments were. ; The top 3 importers of Spectramax are India with 365. The ELISA plates were read at an absorbance of 450 nm on a SpectraMax i3x Plate Reader using Softmax Pro 7. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email drugdiscoverytech@lifetech. application modules • Multi-mode microplate reader and imaging system • Wide dynamic range • StainFree™ cell counting technology • Library of user-configurable applications • Advanced curve fitting and statistical analysis • Cell confluence and cell viability Introduction. HTRF is a proprietary time-resolved fluoresce nce technology that overcomesWith these results, as well as CV values below 6% for all standards, both the SpectraMax i3x and the SpectraMax iD5 readers demonstrate high sensitivity and a wide dynamic range for the IP-One assay. Molecular Devices推出的SpectraMax® i3x 多功能酶标仪除了具有全波长的光吸收、荧光、化学发光和FRET检测功能以外，还兼容了客户端模块化升级功能，用户可以根据需要任何时间随意升级至荧光偏振FP、HTRF、AlphaScreen、Western Blot、上转发光检测、细胞成像和带有注射器. SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE. Use the 3. 0. Specifically, we present neurite. Using the SpectraMax i3x reader, the preconfigured SpectraMax Glo Steady-Luc Reporter assay protocol in SoftMax Pro Software was used to measure luminescence. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any application or budget. Time-resolved fluorescence was measured on the SpectraMax Multi-Mode Microplate Readers (see Table 2 for instrument settings). ai; ImageXpress Pico; IN Carta Image Analysis Software; CLONE SCREENING. Instrument. 7 Figures from 1126 Publications Promega. Replicate values were averaged and imported into Design-Expert 8. 313 reference gain check fail. Assay setup for a 384-well low-volume plate. The calculated LOQ values were 0. SpectraMax i3x offers all the techniques you need to explore cell pathway in a single system. 1 Microplate Data Acquisition & Analysis Software for Windows. Overview of Western Blot Cartridge. (SpectraMax i3/i3x) CatchPoint SimpleStep ELISA (SpectraMax Paradigm) SpectraDrop. Calcium. The Results. The SpectraMax i3 Multi-Mode Detection Platform is a versatile instrument that can perform various assays and measurements on microplates and cuvettes. After 2 days of incubation, LUC and REN activities were measured using a SpectraMax ® i3x Multi-Mode detection platform (Molecular Devices, USA) with a Dual-Luciferase Reporter Assay Kit (Promega. D 1 1. Products : SoftMax Pro Software, SoftMax Pro GxP Software, CellReporterXpress software, General, Screenworks, pCLAMP/AxoScope, GenePix Pro, MetaXpress software. SpectraMax® i3x Multi-Mode Detection Platform; SpectraMax® i3 Multi-Mode Detection Platform; SpectraMax® ID3 Multimode Microplate reader; SpectraMax® ID5 Multimode Microplate reader; SpectraMax® Paradigm® Multi-Mode Microplate Reader; SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. SpectraMax Microplate Reader - Choose from our extensive SpectraMax® line of user-friendly microplate readers. 0 mm hex key to remove the six blue retaining screws that secure the transport bracket (B) to the instrument. The SpectraMax® i3x Multi-Mode Microplate Reader with the SpectraMax® MiniMax™ 300 Imaging Cytometer is equipped with transmitted light (TL), green fluorescence (Ex/Em: 460/541), and red (625/713) fluorescence channels. applications, including dual luciferase and ATP assays. ZERO BIAS - scores, article reviews, protocol conditions and more. SpectraMax® i3x Multi-Mode Detection Platform, Molecular Devices. . In order to calculate a humification index (HIX), spectra were obtained with an excitation wavelength of 254 nm over the emissions waveband of 300–480 nm at increments of 5 nm (Ohno 2002. 3. 12911151) 0. If desired, a high-range standard curve may be prepared from 1 ng/mL to 1 μg/mL, or a low-range standard curve may be prepared from 25 pg/mL to 25 ng/mL. Description 1. . SpectraMax i3x Register or Sign In. 0´ 105 dsD NA (p g/ wel ) R F UFluorescence intensity was measured using a Spectramax i3x plate reader (Molecular Devices) with excitation at 575 nm (bandwidth 9 nm) and emission at 608 nm (bandwidth 15 nm). Time-. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. 과 같은 Parameter를 검사하여 평가할 수 있습니다. Eliminate time-dependent substrate addition steps. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for.